Protocol: Thermophilic Incubation

Fig. 1 – Final coagulation of milk proteins after 8 hours of static incubation at 46°C.

Theory:

Milk is a suspension. By introducing specific bacteria (Lactobacillus) and maintaining a consistent temperature, we convert lactose into lactic acid. This acidity coagulates the proteins, creating structure without mechanical force.

The Substrates (Ingredients):

  • 1 Litre Whole Milk.
  • 2 Tablespoons Active Yoghurt.

The Variables:

  • Target Temp: 43°C – 46°C (110°F – 115°F).
  • Incubation Time: 6 to 12 hours.

The Procedure:

  1. Denature: Heat the milk to 85°C (just before boiling) to restructure the proteins.
  2. Cool: Let the temperature drop to 46°C. This is crucial. Too hot, and you kill the bacteria. Too cold, and they sleep.
  3. Inoculate: Whisk the starter (yoghurt) into the warm milk. Do not over-agitate.
  4. Incubate: Pour into a glass jar. Wrap it in a thick towel and place it in a warm oven (turned off) or a cooler box.
  5. Wait: Close the door. Do not check it. Do not jiggle the jar. Let it sit in the dark for 8 hours.
  6. Set: Move gently to the fridge to halt fermentation.

Notes & Calibration

Density Modulation (Thicker Texture): To achieve higher viscosity (Greek-style), suspend the finished product in a muslin cloth or fine mesh sieve over a bowl. Allow gravity to filter out the whey for 6–12 hours in the refrigerator.

Analog Calibration (No Thermometer): If digital measurement is unavailable, rely on sensory thresholds:

  • Cool limit: The substrate is ready for inoculation when a submerged finger can withstand the temperature for a full 10-second count without recoil.
  • Heat limit: Cease thermal input when steam is visible and micro-bubbles begin to nucleate at the perimeter of the pot.